Biochemical Consultant for Green-Fluorescent Protein, Bioluminescence and Biochemistry of Luminescent Systems

Expertise

• Green-fluorescent protein (GFP): isolation and characterization
• Marine bioluminescence
• Electrophoresis
• GFP in HTS assay development
• Biochemistry of luminescent systems
• Protein and peptide chemistry including isolation and purification
• Batch methods of protein purification such as tangential flow ultra filtration and three-phase partitioning.
• Protein photoinactivation
• HPLC
• Bioluminescent genetic assay development
• Protease assay development
• Assay design and diagnostics kits.

Experience

• Independent Consultant %u2013 biochemical systems
• University Associate Professor, Biochemistry, Department of Biochemistry and Microbiology, since 1977
• Visiting Scientist, Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minn., 1987%u201188
• Associate Professor Biochemistry, Department of Biochemistry and Microbiology

Honors & Publications

• Keynote Speaker: Society for Biomolecular Screening, Biotechnology 2001, Bioluminescence. & Chemiluminescence, Symposium on GFP and Aequorin, Bio-Med Symposium
• Patent: Bioluminescent Indicator Based upon the Expression of a Gene for a Modified Green- Fluorescent Protein and 6 others regarding GFP
• Publications: more than 100
• Books published: three (3) on fluorescent proteins and bioluminescence

Education

• Ph.D., Biology (Biochemistry), Johns Hopkins University
• M.S. , Zoology, University of Florida
• B.S. Ed., Zoology, University of Florida