Protein Biophysics Consultant: Protein Identification and Analysis

Technical Consultant #1317

Merck & Co., West Point, PA 1986-2005

Senior Research Fellow (1994-2005)

Evaluated new protein analytical platforms and performed protein analysis for all proteins submitted for x-ray crystallography.
Purified recombinant proteins for HCV, CMV, HIV programs.
Set up assays for proteases, kinases, and viral enzymes.
Determined solubility properties of all compounds slated for structural studies.
Set-up protein analysis group within Department of Structural Biology improving consistency and timeliness of data to scientists; 2rote SOPs for protein sample submission, use of instruments, and analysis of data.
Introduced and implemented mass spectrophotometry, light scattering, Thermofluor, high-throughput mass spec analysis of proteins improving protein analysis and quality control.
Implemented techniques to purify and crystallize membrane proteins.
Introduced limited proteolysis to identify functional domains in drug targets such as Kinases.
Active member of Research Technologies Review & Licensing Committee.

Research Fellow (1986-1993)

Focused on purifying proteins for various research programs; developed assays for viral proteases and exploratory cancer targets.
Developed assays for inhibitors of blood coagulation.
Set-up screen and counter screens for endothelin converting enzymes.
Identified and purified novel anticoagulant proteins from ticks and hookworms.
Purified and characterized retroviral protease from avian myeloblastosis virus.
Purified and characterized membrane-bound endothelin converting enzyme from cultured cells.
Identified sites of cleavage in p55 GAG by HIV protease.

Harvard Medical School, Boston, MA 1980-1986

Senior Research Associate

Oversaw daily laboratory management, trained new team members, and wrote grants.
Studied Proteases and Protein breakdown, Laboratory Alfred L. Goldberg, Department of Physiology and Biophysics.

University of California, Davis, CA 1976-1979

Research Associate and Lecturer (1977-1979)

Carried out structural analysis of protein kinases, Laboratory of Donal A. Walsh, Department of Biological Chemistry. Supported by fellowship from NIH.

Postdoctoral Fellow (1976-1977)

Purified and characterized protein kinases, Laboratory of Edwin G. Krebs, Department of Biological Chemistry. Supported by fellowship from Muscular Dystrophy Association.

Harvard University, Cambridge, MA 1974-1976

Postdoctoral Fellow

Analyzed phoshoproteins in red blood cell membranes, Laboratory of Dr. Guido Guidotti, Department of Biochemistry. Supported by fellowship from American Leukemia Society.

Academic Work Experience

Lecturer in Biochemistry, U.C. Davis Medical School, Davis, CA
Teaching Fellow in Biochemistry, Harvard University, Cambridge, MA
Teaching Fellow in Biology, Harvard University, Cambridge, MA
Research Assistant in Physics, Temple University, Philadelphia, PA
Undergraduate Teaching Assistant in Physics, Temple University, Philadelphia, PA

Twenty-five publications

Fur patents

PhD., Biophysics, Harvard University, Cambridge, MA

M.A., Physics, Temple University, Philadelphia, PA

B.A., Physics, Temple University, Philadelphia, PA