Molecular Biology Consultant: Cell-based Assays, High Throughput Screening, Target Identification, Gene Therapy and Design of Cell- and Molecular Biology Laboratories

Expertise

• Consultant on biotechnology lab automation, high throughput screening and biological assay development.
• Consultant and expert in design and development of high content cell based assays for high throughput screening.
• Development of non-viral gene delivery techniques.
• Expertise in virology, primarily retrovirus and adenovirus.
• Embryonic stem cell techniques including high Content screening .
• Expert and consultant in the areas of automated and regular microscopy and image analysis.
• Consultant for laboratory automation and design of custom made cell culture systems.
• Teaching and consulting expertise in gene mapping, molecular genetics, applied virology, digital microscopy gene therapy and restriction fragment length polymorphism (or RFLP).
• Successful in establishing a fully integrated center for High Through-Put screening in a multi-disciplinary environment with the capacity to screen and analyze up to 15,000,000 samples/year.
• Expert and consultant in gene delivery methodology, transfection and transduction.
• Expert in Polymerase Chain Reaction (PCR) methodology.
• Designed systems for high throughput cloning and production of lenti and adeno virus based systems.
• Consultant on onjugation of functional peptides and proteins to nucleic acids.
• Expert in establishing transgenic clones.
• Consulting in designing GMP compliant gene therapy laboratories automated with high throughput screening technology.
• Expert in establishing B-cell lines from both healthy donors and immune-compromised patients.
• Expert in murine bone marrow transplantation and murine bone marrow extraction.
• Experienced in nuclear injection/transfection both manual and automated modes.

Experience

Associate Director, University Genome Center 2004 - Present

• Influences and guides the activities at the Genome Center on an executive level.
• Managed to establish University Genome Center with vendors and industry partners to generate a $7,000,000 screening center from a $1,500,000 original investment.
• Directly controls and guides biological lab operations and strategic planning and execution of long-term projects.
• Planned and executed the formation of a state-of-the-art high throughput screening facility with a staff of 25 scientists.

Director of Automation, University: High Throughput Screening in Cell Based Assays 2004 - Present

• Designed and planned the automation suit for hight throughput screen lab, including the in-house designed automated cell culture system capable of 4x10E9 cells per batch.

Postdoctoral Research Fellow: Laboratory of Cellular Biochemistry & Biophysics, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, NY 2002 - 2004

• Trouble shooting of a multi-million dollar functional proteomics project aimed at establishing a full genome wide collection of in-house synthesized siRNA's.

Project Manager: Gene delivery, Clinical Research Center, Karolinska Institute 2001 - 2002

• Managed a team of 8 scientists working on a project aimed at develop technologies relating to non-viral gene delivery techniques, medical devices for enhanced transfection/transduction and receptor-ligand interactions.

Co-founder of Avaris AB 2001

• The driving force behind the incorporation of Avaris AB and established the company based on patents generated during and after PhD work.
• Submitted several patents relating to gene transfer and medical devices.

Visiting Scientist, Perkin-Elmer, Cambridge, UK 2001 - 2005

• Established protocols for 4-D visualization of real-time apoptosis in FYK-/FYN-/Btk /- cells.
• Established protocols for 4-D visualization of the infection by attenuated Poliovirus in actin-GFP transfected human cells.

Visiting scientist at Oswel Scientific, Southampton, UK 2000 - 2005

• Established protocols for conjugation of S.M nuclease to Peptide Nucleic Acid (PNA).

Visiting Scientist at Department of Biosciences, Division of Biochemistry, University of Helsinki, Finland 1998 - 2000

• Established the result basis for "BTK-base", database describing the mutations in Brutons Tyrosine Kinase, a cytoplasmic tyrosine kinase.

Visiting Scientist at "Division of Research Immunology and Bone Marrow Transplantation" Children's Hospital of Los Angeles with Donald B. Kohn 1998 - 2006

• Established SOP's for generation of MMLV producing clones.

Visiting Scientist at Umea University

• Established protocols for the characterization of the genomic organization of Brutons Tyrosine kinase, Btk.
• Validated genomic phage libraries for exon-intron characterization of the Btk-gene.

Honors & Publications

Patents and Publications

• Many publications and invited lectures.
• Patents in diverse areas such as gene delivery, medical devices, receptor specificity screening etc.

Memberships

• Swedish Medical Society
• Swedish Pharmaceutical Society
• American Society for Gene Therapy
• European Society for Gene Therapy
• Royal Institute of Technology, Confocal Microscopy Society

Education

• PhD, Karolinska Institute, Sweden, 2001 Cell and Molecular Biology, Thesis, "The development of synthetic gene delivery systems."
• MSc, University of Lund, Sweden, 1993, Molecular Genetics

Additional education, PhD or higher level:

• Signal transduction
• Understanding signal transduction
• Understanding eukaryotic gene expression
• Experimental animal management
• Transgenic mice
• Digital microscopy
• GFP and Advanced Microscopy in Cell Biology