Protein Drug Products Expert including Enzymes, Antibodies and Antigens

Technical Consultant #1823

Purification and characterization of proteins and glycoproteins.
Protein and glycoprotein drug products including enzymes, antibodies and antigens.
Enzymology - development of reliable, linear assays (colorimetric, fluorometric, radioactive), kinetic analysis of enzymatic reactions.
Technical writing and editing (e.g., CMC sections of new drug applications, biological licensing agreements; technical reports, white papers, development reports, validation reports).
Miscellaneous separation techniques (e.g., dialysis, ultrafiltration, membrane centrifugation lyophilization).
Immunochemical techniques (e.g., ELISAs, RIAs, blotting analysis, immunoprecipitation, double immunodiffusion).
Chromatographic techniques (e.g., column, HPLC, GLC, HP-TLC).
Electrophoretic techniques (e.g., SDS-PAGE, Native-PAGE, capillary electrophoresis, isoelectric focusing, pulsed field gel electrophoresis).
Centrifugal techniques (e.g., differential, velocity sedimentation, velocity density, Isopycnic).

Undisclosed Company, President, 2010 - Present

Lehigh University, Bethlehem, Pennsylvania, 1982 - Present

Professor of Chemistry Emeritus, 2010 - Present
Professor, Chemistry Department, 1995 - 2009
Professor, Chemistry Department, Head, Biochemical Sciences, and Director, Institute for Health

Sciences, 1989 - 1995

Professor, Chemistry Department and Head, Division of Biochemical Sciences, 1984 - 1989
Professor, Chemistry Department, 1982 - 1984

University of California at San Diego, School of Medicine, La Jolla, CA, 1972 - 1982

N.I.H. Postdoctoral Fellow, Department of Neurosciences, 1972 - 1974

Research Projects

Digestive Care Inc., Characterization of Pancrelipase Proteins.
LESN Research Fund: Sperm Fucosidase Study.
Biochemical Basis of Human Disease Fund.
St. Lukes Hospital: Breast Cancer/Cathepsin D Study and STC Diagnostics,Cathepsin D in node-negative breast cancer patients.
Minister of Science and Education (Spain), purification and characterization of rat sperm.
Pool Health Care Trust: Neurotoxicity of human tissue ribonuclease II N, human tissue ribonuclease isoenzyme glycosylation, surgical oncology and, characterization, identification of a compound enriched in Cystic Fibrosis urine and biochemical markers in cancer patients.
Merck & Co., Inc., Glycosylation of monoclonal antibody and glycosylation of recombinant hepatitis B surface antigen.
National Institutes of Health: Studies on the carbohydrate moieties of ą, A Preparative Ultracentrifuge, and human liver ą-L-Fucosidase and Fucosidosis, and human liver sialytransferase and ą-L-Fucosidase.
Cystic Fibrosis Foundation: Studies on cystic fibrosis tissue ą-D-Glucosidase.
Kroc Foundation: Characterization of altered glycoprotein metabolism in human diabetes.
American Lung Association: Cystic fibrosis - biochemical studies and studies on the biochemical defect in cystic fibrosis.
National Foundation - March of Dimes: Fucosidosis, human ą-L-Fucosidase and Mental Retardation.

Academic and Professional Affiliations

Awards

Publications

Approximately 100 Scientific Publications and 10 Invited Reviews, Chapters of Books, and Monographs.
75 Papers Presented at Scientific Meetings and Universities