Nano Biotechnology: DNA Nanoparticles Interaction Viz Halloysite Nanotubes Expert

Expertise

• Halloysite nanotubes, Nanobiotechnology, DNA nanoparticle interactions, Animal Tissue Culture, Primary Culture, Cell cycle pathways, Cell expression, Protein purification, Process development

Compliance

• Laboratory Quality System and Internal Audit as per ISO/IEC 17025:-Updated Version and NABL Requirements.
• Occupational Health and Safety Management System. (OHSMS 18001).
• Preparation and execution of SOPs.
• Statistical analysis and evaluation of data routine environmental monitoring of TC lab using microbiological load evaluation.
• Trouble shooting in process and equipment.
• GLP and laboratory safety requirements.

Compliance

• Preparation and execution of SOPs.
• Statistical analysis and evaluation of data routine environmental monitoring of TC lab using microbiological load evaluation.
• Trouble shooting in process and equipment.
• GLP and laboratory safety requirements.

Animal Cell Culture and Molecular Biology

• Preparation of cell culture media and sterility testing.
• Maintenance of various mammalian and cancerous cell Lines.
• Primary culture of neonatal as well as adult cardiomyocytes, immune cells (thymocytes and neuronal culture.
• Cell culture process optimization (Revival, Harvesting, Subculture) in various size cell culture vessels including T-25, T-75.
• Cryopreservation and preparation of master and working cell banks.
• Isolation of total RNA and genomic DNA from mammalian cell lines as well as animal tissues.
• RT-PCR amplification for checking expression of desired genes.
• Taqman low density array (macro array).
• Transfection technique and reporter assays like SEAP, β-Gal.
• ELISA'S (Cell and Non cell assays) based on Fluorescence and Luminoscence.

Experience

Summary

• Heading a team in work and research of Halloysite nanotubes resulting in numerous articles authored and published regarding HNTs.
• Recent study entitled 'Halloysite nanotubes - An efficient 'nano-support' for the immobilization of ą-amylase' shows experience in the field of enzyme immobilization on HNTs: Here functionalized HNT has been utilized for the immobilization purpose.
• Carried out a study to understand the conduct of HNT with the DNA intercalator Acridine Orange. The results presented (paper-'Study-behavior of HnT with DNA intercalator Acridine Orange') conclude that the degree of intercalation can be reduced in the presence of the HNT setting.
• The study of Gold nanoparticle (AuNP) and HNT based composite; used to study its interaction with DNA. This study confirmed the adsorption of DNA on HNT-AuNPs composites. (Paper Authored) 'Interaction - (DNA) with Halloysite Nanotube (Gold Nanoparticle composites)' presented the possibility of using HNT as support matrices for the attachment of AuNPs and its further use in biosensor development
• Similar study of silver nanoparticles-HNT composite, to detect the DNA damage; showed a possibility of using AgNP-HNT composite for enhancement of shelf life of AgNPs along with its further applications in biological and medical fields.

Undisclosed University, 2009 - Present

• Directorate of Forensic Science
• Presently working as Assistant Professor of the Biology Nano Technology Department.

Torrent Research Centre, Ahmedabad, India, 2005 - 2009

• Scientist for the Cellular and Molecular Biology Department.

USV Limited, Mumbai, India, 2004 - 2005

• Research Associate with focus to tissue culture for the Molecular Medicine Research Laboratory.

Industrial Toxicology Research Centre, 2003 - 2004

• Junior Research Fellow (JRF) in Department of Immunotoxicology.

Maharishi Arvind College of Science and Management, Jaipur India, 2001

• Lecturer for the Department of Pharmacy.

Accomplishments

• Molecular toxicology - Apoptosis and Necrosis in acute Cadmium intoxication.
• Process development for the Lab-scale production of a nutraceuticals.
• PHB production from Halophilic bacteria.
• Protein purification techniques: Dialysis, ammonium sulphate precipitation, SDS PAGE, Western Transfer, TFF, AKTA biochemical and enzyme based assay. Bacterial Transformation, DNA ligation and Cloning Techniques.
• Analytical and bioanalytical techniques: GC-HS, Scanning Electron Microscope, HPLC, SCF, Smart HPLC, Differential Scanning Calorimetry.
• FT-IR, Raman, Zetasizer, GC-HS, Scanning Electron Microscope, HPLC, SCF, Smart HPLC, and Differential Scanning Calorimetry.

Projects

• Undertook project work on Molecular toxicology: Apoptosis and Necrosis in acute Cadmium intoxication-An in vivo and in vitro study at Industrial Toxicology Research Centre,
• Process development for the Lab-scale production of a nutraceutical at Birla Institute of Technology and Science.
• An industrial biotechnology project: PHB production from Halophilic bacteria at Birla Institute of Scientific Research.

Honors & Publications

Credentials

• Certificate Course in Computer Application from Govt. Polytechnic, Jaipur
• C, C++ programming, UNIX and MS office

Awards

• Awarded CSIR'S GATE Fellowship
• Awarded MHRD GATE Fellowship (Qualified in GATE)

Publications

Author-various topics

• Matrices in Halloysite
• Halloysite Nano Tubes-Applications
• Halloysite nanotubes relating to nanocomposites
• DNA , Halloysite nanotube-gold nanoparticle (composite-interactions)
• Malachite Green (adsorption matrices)
• Methotrexate, DNA Gold Nanoparticles (probe-analysis)

Workshops Conferences and Lectures

• International conference of Forensic nanotechnology
• Workshop on Nano HPLC
• Application of nanotechnology and biotechnology in drug delivery systems-Emerging trends of Halloysite Nanotubes