Protein Engineering, Purification, Mutagenesis and Cloning Biochemistry Expert

Technical Consultant #2407

Molecular biology techniques; PCR, mutagenesis and cloning.
Expression of heterologous proteins in variety of organisms such as bacteria, yeast, insect and mammalian cells.
Protein expression analysis using High-Throughput Screening.
Evaluation of protein expression for solubility, stability and scalability using numerous tags (example: His-tag, MBP-tag, Halo-tag etc.).
Method development and implementation (microgram to gram quantities) of protein purification processes from recombinant sources using almost all types of chromatography techniques.
Purification of challenging proteins such as antibodies, membrane proteins and transcription factors.
New and state of the art protein characterization technologies.
Design, planning and execution of projects to obtain reagents such as novel proteases that lead to specificity and cost saving measures.
Protein engineering and structure solving using protein X-ray crystallography.
Discovery based proteomics using Mass Spectrometry and identification and validation of Post-Translational Modifications (PTMs).
Assay development and enzymology.
Lab organization and management.
CD spectroscopy, SEC-MALS, FPLC, HPLC, MST, liquid handlers.

Specific Focus

Independent Consultant, Present

DowDuPont, Johnston, IA, Research Scientist

Initiate, design, execute and analyze scientific projects in the department of Analytical Genomics and Technology.
Establish new protein engineering, purification and characterization methodologies.
Manage and train research personnel in protein expression, purification and characterization.
Interact and collaborate with project managers, principal investigators, scientists, CRO and research associates to conceive, lead, analyze and provide protein purification and characterization support.
Lead research projects and project meetings.

Stowers Institute for Medical Research, Kansas City, MO

Postdoctoral Research Fellow

Initiate, design, execute and analyze scientific projects in the field of proteomics and biochemistry.
Establish custom assays and purification protocols.
Train undergraduate and graduate students in proteomics and biochemistry.
Interact and collaborate with account managers, principal investigator, scientists, programmers and technicians to conceive, lead, analyze, prepare and publish scientific data, abstracts, papers, posters etc. in meetings and prestigious journals.

University of Bayreuth, Bayreuth, Germany, Ph.D. Student

Initiate, design, execute and analyze scientific projects in the field of structural biology and biochemistry.
Develop and establish mass spectrometry and biochemistry based assays for enzymology and small molecule based drug discovery.
Mentor undergraduate and graduate students in structural biology, biophysics and biochemistry.
Prepare and publish scientific data, abstracts, papers, posters etc. in meetings and prestigious journals.

University of Nebraska-Lincoln, Lincoln, NE, Research Manager and Technician

Initiate, design, execute and analyze scientific projects in the field of structural biology and biochemistry.
Manager/supervised undergraduate and graduate students in the fields of structural biology and biochemistry.
Provide day to day technical and managerial support to the laboratory.
Help principal investigator with budgeting and procurement of laboratory supplies.
Prepare and review protocols, SOPs, chemical and MSDS lists for laboratory.

Credentials

Ph.D. program in Biochemistry and IMPRS program, Max-Planck Institute, Dortmund, Germany.
Crystallography software ranging from data collection (mardtb), processing (XDS, imosflm), refinement (CCP4, SHELX), modeling (COOT), validation and data visualization (CCP4mg, Pymol etc.).
Mass spectrometry and HPLC based software such as Unicorn, Mascot, SEQUEST and DTASelect.
Windows, Macintosh and Unix operating systems and their associated software such as Microsoft office.

Languages

Academic and Professional Affiliations

Awards

Publications